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miRNA Analysis

Figure #1: Volcano Plot of Differentially Expressed Genes

microRNA sequencing(miRNA-seq), is the use of next-generation sequencing or massively parallel high-throughput DNA sequencing to sequence microRNAs, also called miRNAs. miRNA-seq differs from other forms of RNA-seq in that input material is often enriched for small RNAs.

  1. Step 1. Reads Alignment
  2. Step 2. Quantify known and novel micro RNA (miRNA) from small RNA sequencing (RNAseq).
  3. Step 3. Statistical analysis: estimate variance-mean dependence in miRNA count data and test for differential expression.
  4. Step 4. miRNA expression Analysis.
  5. Step 5. Normalization of count datasets.
  6. Step 6. Samples clustering.
  7. Step 7. Target Gene prediction
  8. Step 8. Functional Annotation (GO and pathway analysis)

****Receive full package: mapped bam files, FastQC quality insight, count expression table, and differential miRNAs analysis, and targeted genes annotation.