Contact Contact Us
Contact Us

ChIP-Seq Analysis

How ChIP-seq data analysis works

Chromatin immunoprecipitation coupled with sequencing (ChIP-seq) is a genomics and epigenomics method used to study DNA-protein interactions. ChIP-Seq analysis can provide information about the chromatin state and transcription factor binding in a determined gene or loci.

Step 1 - Preprocess Reads
Step 2 - Quality Control Analysis
Step 3 - Mapping
Step 4 - Aligned Reads Quality Check
Step 5 - Peak Calling
  • Fragment estimation
  • Peak identification
  • Table with peak information such as enrichment score, -log10p-value, -log10q-value and the position to peak start.
Step 6 - Annotation
  • KEGG Pathways
  • GO Analysis
Step 7 - Visualization
  • Heatmaps
  • Enrichment plot
  • upSet and coverage plot